Human sE-Selectin / CD62E ELISA Kit PicoKine®

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) . A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 150 149 147 149 1.8 1.2% Sample 2 1179 1233 1221 1130 1190 40.4 3.3% Sample 3 5147 5002 5824 5904 5469 399.06 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 150 149 147 149 1.8 1.2% Sample 1 152 150 149 147 149 1.8 1.2% Sample 2 1179 1233 1221 1130 1190 40.4 3.3% Sample 2 1179 1233 1221 1130 1190 40.4 3.3% Sample 3 5147 5002 5824 5904 5469 399.06 7.2% Sample 3 5147 5002 5824 5904 5469 399.06 7.2%

E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; SELE; ELAM1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 152 1179 5147 138 1081 5271 Standard deviation 12 70.74 303.67 11.04 76.75 390.05 CV (%) 7.9% 6% 5.9% 8% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 152 1179 5147 138 1081 5271 Standard deviation 12 70.74 303.67 11.04 76.75 390.05 CV (%) 7.9% 6% 5.9% 8% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 152 1179 5147 138 1081 5271 Mean (pg/ml) 152 1179 5147 138 1081 5271 Standard deviation 12 70.74 303.67 11.04 76.75 390.05 Standard deviation 12 70.74 303.67 11.04 76.75 390.05 CV (%) 7.9% 6% 5.9% 8% 7.1% 7.4% CV (%) 7.9% 6% 5.9% 8% 7.1% 7.4%

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.105 0.16 0.287 0.514 0.903 1.463 2.077 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.105 0.16 0.287 0.514 0.903 1.463 2.077 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.105 0.16 0.287 0.514 0.903 1.463 2.077 O.D. 0.015 0.105 0.16 0.287 0.514 0.903 1.463 2.077

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.