Rat Rantes ELISA Kit PicoKine®

Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 30 33 31 1.78 5.7% Sample 2 208 182 192 203 196 10.05 5.1% Sample 3 374 383 389 374 380 6.36 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 30 33 31 1.78 5.7% Sample 1 34 30 30 33 31 1.78 5.7% Sample 2 208 182 192 203 196 10.05 5.1% Sample 2 208 182 192 203 196 10.05 5.1% Sample 3 374 383 389 374 380 6.36 1.6% Sample 3 374 383 389 374 380 6.36 1.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 208 374 37 194 349 Standard deviation 2.21 15.8 19.82 2.81 14.93 27.2%2 CV (%) 6.5% 7.6% 5.3% 7.6% 7.7% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 208 374 37 194 349 Standard deviation 2.21 15.8 19.82 2.81 14.93 27.2%2 CV (%) 6.5% 7.6% 5.3% 7.6% 7.7% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 208 374 37 194 349 Mean (pg/ml) 34 208 374 37 194 349 Standard deviation 2.21 15.8 19.82 2.81 14.93 27.2%2 Standard deviation 2.21 15.8 19.82 2.81 14.93 27.2%2 CV (%) 6.5% 7.6% 5.3% 7.6% 7.7% 7.2% CV (%) 6.5% 7.6% 5.3% 7.6% 7.7% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.124 0.207 0.373 0.676 1.137 1.75 2.083 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.124 0.207 0.373 0.676 1.137 1.75 2.083 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.124 0.207 0.373 0.676 1.137 1.75 2.083 O.D. 0.045 0.124 0.207 0.373 0.676 1.137 1.75 2.083

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.