Human PLGF/PGF ELISA Kit PicoKine®

Human PLGF ELISA Kit PicoKine® (96 Tests) . Quantitate Human PGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The protein, also called PGF, is 149 amino acids long and shares 53% identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 37 42 39 1.92 4.9% Sample 2 221 250 264 244 244 15.51 6.3% Sample 3 454 469 445 509 469 24.49 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 37 42 39 1.92 4.9% Sample 1 40 38 37 42 39 1.92 4.9% Sample 2 221 250 264 244 244 15.51 6.3% Sample 2 221 250 264 244 244 15.51 6.3% Sample 3 454 469 445 509 469 24.49 5.2% Sample 3 454 469 445 509 469 24.49 5.2%

Synonyms

Placenta growth factor; PlGF; PGF; PGFL, PLGF

Gene Name

PGF

Gene ID

5228

UniProt

P49763

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-R149

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

Applications

ELISA

Field of Research

Cancer, Cancer Metabolism, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 221 454 37 215 459 Standard deviation 3.16 13.26 23.15 3.51 13.76 25.7 CV (%) 7.9% 6% 5.6% 9.5% 6.4% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 221 454 37 215 459 Standard deviation 3.16 13.26 23.15 3.51 13.76 25.7 CV (%) 7.9% 6% 5.6% 9.5% 6.4% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 221 454 37 215 459 Mean (pg/ml) 40 221 454 37 215 459 Standard deviation 3.16 13.26 23.15 3.51 13.76 25.7 Standard deviation 3.16 13.26 23.15 3.51 13.76 25.7 CV (%) 7.9% 6% 5.6% 9.5% 6.4% 5.6% CV (%) 7.9% 6% 5.6% 9.5% 6.4% 5.6%

Sensitivity

<1 pg/mL

Function

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24789 MW

Specificity

Natural and recombinant human PLGF

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Placenta growth factor

Subcellular Location

Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

Protein Name

Placenta growth factor

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.139 0.219 0.378 0.609 1.024 1.547 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.139 0.219 0.378 0.609 1.024 1.547 2.098 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.139 0.219 0.378 0.609 1.024 1.547 2.098 O.D. 0.042 0.139 0.219 0.378 0.609 1.024 1.547 2.098

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.