Rat PDGF-AB ELISA Kit PicoKine®

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV) . It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 83 85 86 85 1.22 1.4% Sample 2 282 277 283 282 281 2.34 0.8% Sample 3 885 834 857 888 866 22.07 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 83 85 86 85 1.22 1.4% Sample 1 86 83 85 86 85 1.22 1.4% Sample 2 282 277 283 282 281 2.34 0.8% Sample 2 282 277 283 282 281 2.34 0.8% Sample 3 885 834 857 888 866 22.07 2.5% Sample 3 885 834 857 888 866 22.07 2.5%

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Immunology, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted Molecules, Serum Proteins, Signal Transduction, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 282 885 83 277 930 Standard deviation 4.9 14.38 58.41 4.98 16.89 74.4 CV (%) 5.7% 8%% 6.6% 6% 6.1% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 282 885 83 277 930 Standard deviation 4.9 14.38 58.41 4.98 16.89 74.4 CV (%) 5.7% 8%% 6.6% 6% 6.1% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 282 885 83 277 930 Mean (pg/ml) 86 282 885 83 277 930 Standard deviation 4.9 14.38 58.41 4.98 16.89 74.4 Standard deviation 4.9 14.38 58.41 4.98 16.89 74.4 CV (%) 5.7% 8%% 6.6% 6% 6.1% 8% CV (%) 5.7% 8%% 6.6% 6% 6.1% 8%

Pdgfa: Growth factor that plays an essential role in theregulation of embryonic development, cell proliferation, cellmigration, survival and chemotaxis. Potent mitogen for cells ofmesenchymal origin. Required for normal lung alveolar septumformation during embryogenesis, normal development of thegastrointestinal tract, normal development of Leydig cells andspermatogenesis. Required for normal oligodendrocyte developmentand normal myelination in the spinal cord and cerebellum. Plays animportant role in wound healing. Signaling is modulated by theformation of heterodimers with PDGFB (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.081 0.173 0.251 0.391 0.63 1.092 1.831 2.965 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.081 0.173 0.251 0.391 0.63 1.092 1.831 2.965 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.081 0.173 0.251 0.391 0.63 1.092 1.831 2.965 O.D. 0.081 0.173 0.251 0.391 0.63 1.092 1.831 2.965

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.