Human OPN / Osteopontin ELISA Kit PicoKine®

Human OPN / Osteopontin ELISA Kit PicoKine® (96 Tests) . Quantitate Human SPP1 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1) . Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 305 309 272 322 302 18.42 6% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 3 4985 4178 4476 4405 4511 294.96 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 305 309 272 322 302 18.42 6% Sample 1 305 309 272 322 302 18.42 6% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 2 1771 1751 1709 1739 1742 22.46 1.2% Sample 3 4985 4178 4476 4405 4511 294.96 6.5% Sample 3 4985 4178 4476 4405 4511 294.96 6.5%

Synonyms

Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP, OPN; PSEC0156

Gene Name

SPP1

Gene ID

6696

UniProt

P10451

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-N300

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Bone. Found in plasma.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 305 1771 4985 314 1611 5408 Mean (pg/ml) 305 1771 4985 314 1611 5408 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 Standard deviation 14.03 138.13 304.08 16.64 133.71 383.96 CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1% CV (%) 4.6% 7.8% 6.1% 5.3% 8.3% 7.1%

Sensitivity

<10 pg/mL

Function

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35423 MW

Specificity

Natural and recombinant human OPN

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Osteopontin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the osteopontin family.

Protein Name

Osteopontin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389 O.D. 0.022 0.09 0.149 0.266 0.524 1.056 1.901 2.389

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.