Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 142 137 133 136 3.93 2.8% Sample 2 650 625 651 585 627 26.78 4.2% Sample 3 2696 3024 2657 2825 2800 143.23 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 142 137 133 136 3.93 2.8% Sample 1 132 142 137 133 136 3.93 2.8% Sample 2 650 625 651 585 627 26.78 4.2% Sample 2 650 625 651 585 627 26.78 4.2% Sample 3 2696 3024 2657 2825 2800 143.23 5.1% Sample 3 2696 3024 2657 2825 2800 143.23 5.1%

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Metabolism, Obesity, Signal Transduction, Structures, TNF Superfamily, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 650 2696 130 692 2678 Standard deviation 10.42 37.7 113.23 11.57 51.2 136.57 CV (%) 7.9% 5.8% 4.2% 8.9% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 650 2696 130 692 2678 Standard deviation 10.42 37.7 113.23 11.57 51.2 136.57 CV (%) 7.9% 5.8% 4.2% 8.9% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 650 2696 130 692 2678 Mean (pg/ml) 132 650 2696 130 692 2678 Standard deviation 10.42 37.7 113.23 11.57 51.2 136.57 Standard deviation 10.42 37.7 113.23 11.57 51.2 136.57 CV (%) 7.9% 5.8% 4.2% 8.9% 7.4% 5.1% CV (%) 7.9% 5.8% 4.2% 8.9% 7.4% 5.1%

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.051 0.09 0.193 0.406 0.726 1.465 1.897 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.051 0.09 0.193 0.406 0.726 1.465 1.897 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.014 0.051 0.09 0.193 0.406 0.726 1.465 1.897 O.D. 0.014 0.051 0.09 0.193 0.406 0.726 1.465 1.897

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.