Human NT-4/NTF4/Neurotrophin-4 ELISA Kit PicoKine®

Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons (2-4) . NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 99 93 89 93 3.56 3.8% Sample 2 234 212 207 213 216 10.35 4.7% Sample 3 888 924 870 895 894 19.44 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 99 93 89 93 3.56 3.8% Sample 1 94 99 93 89 93 3.56 3.8% Sample 2 234 212 207 213 216 10.35 4.7% Sample 2 234 212 207 213 216 10.35 4.7% Sample 3 888 924 870 895 894 19.44 2.1% Sample 3 888 924 870 895 894 19.44 2.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 234 888 93 224 873 Standard deviation 6.01 13.33 69.26 7.8%5 15.45 84.68 CV (%) 6.4% 5.7% 7.8% 6.4% 6.9% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 234 888 93 224 873 Standard deviation 6.01 13.33 69.26 7.8%5 15.45 84.68 CV (%) 6.4% 5.7% 7.8% 6.4% 6.9% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 234 888 93 224 873 Mean (pg/ml) 94 234 888 93 224 873 Standard deviation 6.01 13.33 69.26 7.8%5 15.45 84.68 Standard deviation 6.01 13.33 69.26 7.8%5 15.45 84.68 CV (%) 6.4% 5.7% 7.8% 6.4% 6.9% 9.7% CV (%) 6.4% 5.7% 7.8% 6.4% 6.9% 9.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.059 0.085 0.139 0.277 0.534 1.188 2.278 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.059 0.085 0.139 0.277 0.534 1.188 2.278 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.059 0.085 0.139 0.277 0.534 1.188 2.278 O.D. 0.031 0.059 0.085 0.139 0.277 0.534 1.188 2.278

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.