Rat Neurotrophin-3 ELISA Kit PicoKine®

Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 38 39 39 39 1.08 2.7% Sample 2 173 159 176 168 169 6.44 3.8% Sample 3 464 416 419 479 444 27.53 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 38 39 39 39 1.08 2.7% Sample 1 41 38 39 39 39 1.08 2.7% Sample 2 173 159 176 168 169 6.44 3.8% Sample 2 173 159 176 168 169 6.44 3.8% Sample 3 464 416 419 479 444 27.53 6.2% Sample 3 464 416 419 479 444 27.53 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 41 173 464 41 177 504 Mean (pg/ml) 41 173 464 41 177 504 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 Standard deviation 2.25 12.45 23.66 2.99 15.53 27.72 CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5% CV (%) 5.5% 7.2% 5.5% 7.3% 9% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042 O.D. 0.05 0.132 0.204 0.374 0.645 1.118 1.865 2.042

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.