Human Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine®

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 52 40 60 51 2.29 4.5% Sample 2 99 89 107 76 93 3.79 4.1% Sample 3 673 660 887 1000 805 33.97 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 52 40 60 51 2.29 4.5% Sample 1 50 52 40 60 51 2.29 4.5% Sample 2 99 89 107 76 93 3.79 4.1% Sample 2 99 89 107 76 93 3.79 4.1% Sample 3 673 660 887 1000 805 33.97 4.2% Sample 3 673 660 887 1000 805 33.97 4.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 99 673 57 106 839 Standard deviation 1.05 5.62 40.65 3.51 8.14 56.13 CV (%) 2.1% 5.7% 6% 6.2% 7.7% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 99 673 57 106 839 Standard deviation 1.05 5.62 40.65 3.51 8.14 56.13 CV (%) 2.1% 5.7% 6% 6.2% 7.7% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 99 673 57 106 839 Mean (pg/ml) 50 99 673 57 106 839 Standard deviation 1.05 5.62 40.65 3.51 8.14 56.13 Standard deviation 1.05 5.62 40.65 3.51 8.14 56.13 CV (%) 2.1% 5.7% 6% 6.2% 7.7% 6.7% CV (%) 2.1% 5.7% 6% 6.2% 7.7% 6.7%

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.149 0.203 0.291 0.467 0.753 1.141 1.803 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.149 0.203 0.291 0.467 0.753 1.141 1.803 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.149 0.203 0.291 0.467 0.753 1.141 1.803 O.D. 0.065 0.149 0.203 0.291 0.467 0.753 1.141 1.803

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.