Mouse MMP-9 ELISA Kit PicoKine®

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9) . The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 213 210 203 207 3.96 1.9% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 3 4289 3856 3877 4024 4011 172.79 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 213 210 203 207 3.96 1.9% Sample 1 205 213 210 203 207 3.96 1.9% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 2 1787 1777 1813 1705 1770 40.03 2.2% Sample 3 4289 3856 3877 4024 4011 172.79 4.3% Sample 3 4289 3856 3877 4024 4011 172.79 4.3%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 205 1787 4289 222 1732 4214 Mean (pg/ml) 205 1787 4289 222 1732 4214 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 Standard deviation 15.37 141.17 214.45 18.64 143.75 214.91 CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1% CV (%) 7.5% 7.9% 5% 8.4% 8.3% 5.1%

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.