Human MMP-9 ELISA Kit PicoKine®

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9) . The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 203 233 214 221 13.88 6.2% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 203 233 214 221 13.88 6.2% Sample 1 237 203 233 214 221 13.88 6.2% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 2 1530 1745 1683 1555 1628 88.94 5.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4% Sample 3 5547 5421 5245 5758 5492 186.96 3.4%

Matrix metalloproteinase-9; MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase; Gelatinase B; GELB;67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9; MMP9; CLG4B

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 237 1530 5547 250 1429 5731 Mean (pg/ml) 237 1530 5547 250 1429 5731 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 Standard deviation 18.72 108.63 439.41 23.5 135.75 504.32 CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8% CV (%) 7.9% 7.1% 7.9% 9.4% 9.5% 8.8%

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469 O.D. 0.031 0.094 0.177 0.315 0.587 1.032 1.701 2.469

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.