Human MMP-7/Matrilysin ELISA Kit PicoKine®

Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively) . Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 250 279 270 12.09 4.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 3 5090 5484 5287 4635 5124 314.81 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 250 279 270 12.09 4.4% Sample 1 272 280 250 279 270 12.09 4.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 2 1042 1051 1050 1108 1062 26.35 2.4% Sample 3 5090 5484 5287 4635 5124 314.81 6.1% Sample 3 5090 5484 5287 4635 5124 314.81 6.1%

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1042 5090 256 1008 5032 Mean (pg/ml) 272 1042 5090 256 1008 5032 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 Standard deviation 12.51 43.76 336.64 15.36 47.37 412.62 CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2% CV (%) 4.6% 4.2% 6.6% 6% 4.7% 8.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326 O.D. 0.01 0.072 0.176 0.348 0.576 1.135 1.829 2.326

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.