Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®

Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 281 283 271 287 280 5.89 2.1% Sample 2 1427 1569 1617 1589 1550 73.31 4.7% Sample 3 4432 4951 4788 4684 4713 188.46 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 281 283 271 287 280 5.89 2.1% Sample 1 281 283 271 287 280 5.89 2.1% Sample 2 1427 1569 1617 1589 1550 73.31 4.7% Sample 2 1427 1569 1617 1589 1550 73.31 4.7% Sample 3 4432 4951 4788 4684 4713 188.46 3.9% Sample 3 4432 4951 4788 4684 4713 188.46 3.9%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1427 1427 4432 300 1524 4802 Standard deviation 17.14 68.49 230.46 21 102.1 288.12 CV (%) 6.1% 4.8% 5.2% 7% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1427 1427 4432 300 1524 4802 Standard deviation 17.14 68.49 230.46 21 102.1 288.12 CV (%) 6.1% 4.8% 5.2% 7% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1427 1427 4432 300 1524 4802 Mean (pg/ml) 1427 1427 4432 300 1524 4802 Standard deviation 17.14 68.49 230.46 21 102.1 288.12 Standard deviation 17.14 68.49 230.46 21 102.1 288.12 CV (%) 6.1% 4.8% 5.2% 7% 6.7% 6% CV (%) 6.1% 4.8% 5.2% 7% 6.7% 6%

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.11 0.159 0.264 0.433 0.723 1.284 1.958 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.11 0.159 0.264 0.433 0.723 1.284 1.958 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.11 0.159 0.264 0.433 0.723 1.284 1.958 O.D. 0.051 0.11 0.159 0.264 0.433 0.723 1.284 1.958

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.