Human MMP-3/Stromelysin-1 ELISA Kit PicoKine®

Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 284 300 300 255 284 18.37 6.4% Sample 2 1539 1572 1675 1602 1597 50.24 3.1% Sample 3 4656 3964 4552 4507 4419 268.61 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 284 300 300 255 284 18.37 6.4% Sample 1 284 300 300 255 284 18.37 6.4% Sample 2 1539 1572 1675 1602 1597 50.24 3.1% Sample 2 1539 1572 1675 1602 1597 50.24 3.1% Sample 3 4656 3964 4552 4507 4419 268.61 6% Sample 3 4656 3964 4552 4507 4419 268.61 6%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 284 1539 4656 310 1656 4415 Standard deviation 14.48 100.03 297.98 19.22 112.6 304.63 CV (%) 6.9% 6.5% 6.4% 6.2% 6.8% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 284 1539 4656 310 1656 4415 Standard deviation 14.48 100.03 297.98 19.22 112.6 304.63 CV (%) 6.9% 6.5% 6.4% 6.2% 6.8% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 284 1539 4656 310 1656 4415 Mean (pg/ml) 284 1539 4656 310 1656 4415 Standard deviation 14.48 100.03 297.98 19.22 112.6 304.63 Standard deviation 14.48 100.03 297.98 19.22 112.6 304.63 CV (%) 6.9% 6.5% 6.4% 6.2% 6.8% 6.9% CV (%) 6.9% 6.5% 6.4% 6.2% 6.8% 6.9%

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.05 0.082 0.145 0.26 0.521 0.995 1.902 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.05 0.082 0.145 0.26 0.521 0.995 1.902 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.05 0.082 0.145 0.26 0.521 0.995 1.902 O.D. 0.01 0.05 0.082 0.145 0.26 0.521 0.995 1.902

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.