Human MMP-2 ELISA Kit PicoKine®

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2) . It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch) . The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1326 1365 1368 1233 1323 54.53 4.1% Sample 2 3925 3610 3426 3883 3711 204.2 5.5% Sample 3 14099 16330 14083 16849 15340 1262.66 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1326 1365 1368 1233 1323 54.53 4.1% Sample 1 1326 1365 1368 1233 1323 54.53 4.1% Sample 2 3925 3610 3426 3883 3711 204.2 5.5% Sample 2 3925 3610 3426 3883 3711 204.2 5.5% Sample 3 14099 16330 14083 16849 15340 1262.66 8.2% Sample 3 14099 16330 14083 16849 15340 1262.66 8.2%

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Extracellular Matrix, Invasion/Microenvironment, Metabolism, Metabolism Processes, Metalloprotease, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Response To Hypoxia, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1326 3925 14099 1253 3970 14093 Standard deviation 80.88 282.6 690.85 76.43 349.36 690.55 CV (%) 6.1% 7.2% 4.9% 6.1% 8.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1326 3925 14099 1253 3970 14093 Standard deviation 80.88 282.6 690.85 76.43 349.36 690.55 CV (%) 6.1% 7.2% 4.9% 6.1% 8.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1326 3925 14099 1253 3970 14093 Mean (pg/ml) 1326 3925 14099 1253 3970 14093 Standard deviation 80.88 282.6 690.85 76.43 349.36 690.55 Standard deviation 80.88 282.6 690.85 76.43 349.36 690.55 CV (%) 6.1% 7.2% 4.9% 6.1% 8.8% 4.9% CV (%) 6.1% 7.2% 4.9% 6.1% 8.8% 4.9%

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.06 0.133 0.201 0.336 0.573 1.052 1.535 2.12 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.06 0.133 0.201 0.336 0.573 1.052 1.535 2.12 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.06 0.133 0.201 0.336 0.573 1.052 1.535 2.12 O.D. 0.06 0.133 0.201 0.336 0.573 1.052 1.535 2.12

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.