Human MMP-1 ELISA Kit PicoKine®

Human MMP-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MMP1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% Sample 3 4510 5168 4653 4926 4814 253.07 5.2%

Synonyms

CDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7)

Gene Name

MMP1

Gene ID

4312

UniProt

B4DN15

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F20-N469

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Signal Transduction, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 215 1557 4510 235 1708 4065 Mean (pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6% CV (%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6%

Sensitivity

<20 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

46266 MW

Specificity

Natural and recombinant human total MMP-1

Anticoagulant

Heparin

Gene Name Synonym

Matrix metallopeptidase 1 (interstitial collagenase)

Protein Name

Interstitial collagenase

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998 O.D. 0.009 0.071 0.123 0.22 0.403 0.776 1.389 1.998

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.