Human CCL19/MIP-3 Beta ELISA Kit PicoKine®

Human CCL19/MIP-3 Beta ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine?belonging to the CC chemokine family that is also known as?EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta? (MIP-3-beta) . Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 30 31 29 29 0.82 2.8% Sample 2 234 255 249 222 240 12.9 5.3% Sample 3 421 456 425 465 441 19.07 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 30 31 29 29 0.82 2.8% Sample 1 29 30 31 29 29 0.82 2.8% Sample 2 234 255 249 222 240 12.9 5.3% Sample 2 234 255 249 222 240 12.9 5.3% Sample 3 421 456 425 465 441 19.07 4.3% Sample 3 421 456 425 465 441 19.07 4.3%

Synonyms

C-C motif chemokine ; CCL19 ; hCG_20476

Gene Name

CCL19

Gene ID

6363

UniProt

Q6IBD6

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G22-S98

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 CV (%) 4.8% 6.7% 6.4% 5% 7.5% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 CV (%) 4.8% 6.7% 6.4% 5% 7.5% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 234 421 27 213 398 Mean (pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 CV (%) 4.8% 6.7% 6.4% 5% 7.5% 8.1% CV (%) 4.8% 6.7% 6.4% 5% 7.5% 8.1%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10993 MW

Specificity

Natural and recombinant human CCL19

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 19

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.115 0.234 0.408 0.712 1.234 1.764 1.988 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.115 0.234 0.408 0.712 1.234 1.764 1.988 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.115 0.234 0.408 0.712 1.234 1.764 1.988 O.D. 0.003 0.115 0.234 0.408 0.712 1.234 1.764 1.988

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.