Mouse MIP-2 ELISA Kit PicoKine®

Mouse MIP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cxcl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 34 40 41 38 2.69 7% Sample 2 230 234 204 215 220 11.98 5.4% Sample 3 434 404 419 468 431 23.72 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 34 40 41 38 2.69 7% Sample 1 39 34 40 41 38 2.69 7% Sample 2 230 234 204 215 220 11.98 5.4% Sample 2 230 234 204 215 220 11.98 5.4% Sample 3 434 404 419 468 431 23.72 5.5% Sample 3 434 404 419 468 431 23.72 5.5%

Synonyms

C-X-C motif chemokine 2; Macrophage inflammatory protein 2; MIP2; Cxcl2; Mip-2, Mip2, Scyb2

Gene Name

CXCL2

Gene ID

20310

UniProt

P10889

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A28-N100

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 230 434 37 220 409 Standard deviation 1.79 17.94 17.79 1.85 18.7 22.9 CV (%) 4.6% 7.8% 4.1% 5% 8.5% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 230 434 37 220 409 Standard deviation 1.79 17.94 17.79 1.85 18.7 22.9 CV (%) 4.6% 7.8% 4.1% 5% 8.5% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 230 434 37 220 409 Mean (pg/ml) 39 230 434 37 220 409 Standard deviation 1.79 17.94 17.79 1.85 18.7 22.9 Standard deviation 1.79 17.94 17.79 1.85 18.7 22.9 CV (%) 4.6% 7.8% 4.1% 5% 8.5% 5.6% CV (%) 4.6% 7.8% 4.1% 5% 8.5% 5.6%

Sensitivity

<5 pg/mL

Function

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10621 MW

Specificity

Natural and recombinant mouse MIP-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 2

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.146 0.243 0.439 0.819 1.363 1.972 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.146 0.243 0.439 0.819 1.363 1.972 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.146 0.243 0.439 0.819 1.363 1.972 O.D. 0.044 0.095 0.146 0.243 0.439 0.819 1.363 1.972

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.