Human CCL4/MIP-1 Beta ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 11 13 14 12 1.08 9% Sample 2 90 88 90 93 90 1.78 1.9% Sample 3 254 290 285 250 269 17.89 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 11 13 14 12 1.08 9% Sample 1 13 11 13 14 12 1.08 9% Sample 2 90 88 90 93 90 1.78 1.9% Sample 2 90 88 90 93 90 1.78 1.9% Sample 3 254 290 285 250 269 17.89 6.6% Sample 3 254 290 285 250 269 17.89 6.6%

C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; HC21; Lymphocyte activation gene 1 protein; LAG-1; MIP-1-beta (1-69) ; Macrophage inflammatory protein 1-beta; MIP-1-beta; PAT 744; Protein H400; SIS-gamma; Small-inducible cytokine A4; T-cell activation protein 2; ACT-2; MIP-1-beta (3-69) ; CCL4; LAG1, MIP1B, SCYA4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 90 254 12 95 256 Standard deviation 0.63 4.95 13.2 0.62 6.84 15.87 CV (%) 4.9% 5.5% 5.2% 5.2% 7.2% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 90 254 12 95 256 Standard deviation 0.63 4.95 13.2 0.62 6.84 15.87 CV (%) 4.9% 5.5% 5.2% 5.2% 7.2% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 13 90 254 12 95 256 Mean (pg/ml) 13 90 254 12 95 256 Standard deviation 0.63 4.95 13.2 0.62 6.84 15.87 Standard deviation 0.63 4.95 13.2 0.62 6.84 15.87 CV (%) 4.9% 5.5% 5.2% 5.2% 7.2% 6.2% CV (%) 4.9% 5.5% 5.2% 5.2% 7.2% 6.2%

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) . The processed form MIP-1-beta (3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.044 0.141 0.243 0.419 0.719 1.163 1.712 2.022 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.044 0.141 0.243 0.419 0.719 1.163 1.712 2.022 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.044 0.141 0.243 0.419 0.719 1.163 1.712 2.022 O.D. 0.044 0.141 0.243 0.419 0.719 1.163 1.712 2.022

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.