Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 ly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 17 17 17 0.82 4.8% Sample 2 74 69 76 69 72 3.08 4.2% Sample 3 240 225 228 207 225 11.81 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 19 17 17 17 0.82 4.8% Sample 1 18 19 17 17 17 0.82 4.8% Sample 2 74 69 76 69 72 3.08 4.2% Sample 2 74 69 76 69 72 3.08 4.2% Sample 3 240 225 228 207 225 11.81 5.2% Sample 3 240 225 228 207 225 11.81 5.2%

C-C motif chemokine 3; Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5; Ccl3; Mip1a, Scya3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 74 240 18 69 249 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 CV (%) 4.1% 5.4% 4% 4.2% 7.2% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 74 240 18 69 249 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 CV (%) 4.1% 5.4% 4% 4.2% 7.2% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 74 240 18 69 249 Mean (pg/ml) 18 74 240 18 69 249 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 Standard deviation 0.73 3.99 9.6 0.75 4.96 14.44 CV (%) 4.1% 5.4% 4% 4.2% 7.2% 5.8% CV (%) 4.1% 5.4% 4% 4.2% 7.2% 5.8%

Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.104 0.143 0.234 0.472 0.786 1.364 2.02 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.104 0.143 0.234 0.472 0.786 1.364 2.02 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.045 0.104 0.143 0.234 0.472 0.786 1.364 2.02 O.D. 0.045 0.104 0.143 0.234 0.472 0.786 1.364 2.02

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.