Human MIP-1Alpha/CCL3 ELISA Kit PicoKine®

Human MIP-1Alpha/CCL3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 35 33 1.11 3.3% Sample 2 175 166 172 161 168 5.4 3.2% Sample 3 370 407 390 394 390 13.27 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 35 33 1.11 3.3% Sample 1 33 34 32 35 33 1.11 3.3% Sample 2 175 166 172 161 168 5.4 3.2% Sample 2 175 166 172 161 168 5.4 3.2% Sample 3 370 407 390 394 390 13.27 3.4% Sample 3 370 407 390 394 390 13.27 3.4%

Synonyms

C-C motif chemokine ; CCL3 ; hCG_1749202

Gene Name

CCL3

Gene ID

6348

UniProt

A0N0R1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A27-A92

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 175 370 30 166 361 Standard deviation 2.54 10.32 14.8 2.58 12.45 20.93 CV (%) 7.7% 4% 4% 8.6% 7.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 175 370 30 166 361 Standard deviation 2.54 10.32 14.8 2.58 12.45 20.93 CV (%) 7.7% 4% 4% 8.6% 7.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 175 370 30 166 361 Mean (pg/ml) 33 175 370 30 166 361 Standard deviation 2.54 10.32 14.8 2.58 12.45 20.93 Standard deviation 2.54 10.32 14.8 2.58 12.45 20.93 CV (%) 7.7% 4% 4% 8.6% 7.5% 5.8% CV (%) 7.7% 4% 4% 8.6% 7.5% 5.8%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10085 MW

Specificity

Natural and recombinant human MIP-1 alpha

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

C-C motif chemokine

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 3

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.098 0.144 0.231 0.399 0.768 1.468 2.436 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.098 0.144 0.231 0.399 0.768 1.468 2.436 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.098 0.144 0.231 0.399 0.768 1.468 2.436 O.D. 0.055 0.098 0.144 0.231 0.399 0.768 1.468 2.436

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.