Mouse CCL22/MDC ELISA Kit PicoKine®

Mouse CCL22/MDC ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl22 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22) . MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 54 48 49 3.03 6.1% Sample 2 233 248 234 235 237 6.10 2.5% Sample 3 820 852 936 892 875 43.48 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 54 48 49 3.03 6.1% Sample 1 46 51 54 48 49 3.03 6.1% Sample 2 233 248 234 235 237 6.10 2.5% Sample 2 233 248 234 235 237 6.10 2.5% Sample 3 820 852 936 892 875 43.48 4.9% Sample 3 820 852 936 892 875 43.48 4.9%

Synonyms

C-C motif chemokine 22; Activated B and dendritic cell-derived; CC chemokine ABCD-1; Small-inducible cytokine A22; Ccl22; Abcd1, Scya22

Gene Name

CCL22

Gene ID

20299

UniProt

O88430

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G25-S92

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed by activated splenic B-lymphocytes and dendritic cells. Low expression in lung, thymocytes, lymph node, and unstimulated splenic cells.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 233 820 52 308 871 Standard deviation 2.94 17.48 50.84 4.11 25.26 66.20 CV (%) 6.4% 7.5% 6.2% 7.9% 8.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 233 820 52 308 871 Standard deviation 2.94 17.48 50.84 4.11 25.26 66.20 CV (%) 6.4% 7.5% 6.2% 7.9% 8.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 233 820 52 308 871 Mean (pg/ml) 46 233 820 52 308 871 Standard deviation 2.94 17.48 50.84 4.11 25.26 66.20 Standard deviation 2.94 17.48 50.84 4.11 25.26 66.20 CV (%) 6.4% 7.5% 6.2% 7.9% 8.2% 7.6% CV (%) 6.4% 7.5% 6.2% 7.9% 8.2% 7.6%

Sensitivity

<10 pg/mL

Function

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B- lymphocytes with T-cells in immune responses.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10302 MW

Specificity

Natural and recombinant mouse MDC

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 22

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 22

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.144 0.227 0.368 0.593 0.969 1.616 2.358 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.144 0.227 0.368 0.593 0.969 1.616 2.358 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.144 0.227 0.368 0.593 0.969 1.616 2.358 O.D. 0.013 0.144 0.227 0.368 0.593 0.969 1.616 2.358

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.