Mouse Leptin Receptor ELISA Kit PicoKine®

Mouse Leptin Receptor ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Lepr in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 189 209 209 207 11.79 5.6% Sample 2 1398 1396 1505 1381 1420 49.51 3.4% Sample 3 4930 5654 5332 5633 5387 293.12 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 189 209 209 207 11.79 5.6% Sample 1 222 189 209 209 207 11.79 5.6% Sample 2 1398 1396 1505 1381 1420 49.51 3.4% Sample 2 1398 1396 1505 1381 1420 49.51 3.4% Sample 3 4930 5654 5332 5633 5387 293.12 5.4% Sample 3 4930 5654 5332 5633 5387 293.12 5.4%

Synonyms

Leptin receptor; LEP-R; B219; OB receptor; OB-R; CD295; Lepr; Db, Obr

Gene Name

LEPR

Gene ID

16847

UniProt

P48356

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L22-G839

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform A: highest level of expression in lung and kidney, also present in kidney, heart, brain, spleen, liver, muscle, choroid plexus and hypothalamus. Isoform B: highest level of expression in hypothalamus and lower level in brain, testes and adipose tissue. Isoform E: expressed in adipose tissue, hypothalamus, heart, and testes.

Applications

ELISA

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1398 4930 200 1476 5345 Standard deviation 15.09 86.87 359.89 15.8 110.7 427.6 CV (%) 6.8% 6.1% 7.3% 7.9% 7.5% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1398 4930 200 1476 5345 Standard deviation 15.09 86.87 359.89 15.8 110.7 427.6 CV (%) 6.8% 6.1% 7.3% 7.9% 7.5% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1398 4930 200 1476 5345 Mean (pg/ml) 222 1398 4930 200 1476 5345 Standard deviation 15.09 86.87 359.89 15.8 110.7 427.6 Standard deviation 15.09 86.87 359.89 15.8 110.7 427.6 CV (%) 6.8% 6.1% 7.3% 7.9% 7.5% 8% CV (%) 6.8% 6.1% 7.3% 7.9% 7.5% 8%

Sensitivity

<5 pg/mL

Function

Receptor for obesity factor (leptin) . On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

130789 MW

Specificity

Natural and recombinant mouse Leptin receptor

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Leptin receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Protein Name

Leptin receptor

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.103 0.216 0.432 0.821 1.514 2.179 2.538 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.103 0.216 0.432 0.821 1.514 2.179 2.538 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.103 0.216 0.432 0.821 1.514 2.179 2.538 O.D. 0.006 0.103 0.216 0.432 0.821 1.514 2.179 2.538

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.