Human Leptin ELISA Kit PicoKine®

Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 83 84 87 83 2.16 2.6% Sample 2 485 539 463 476 490 28.93 5.9% Sample 3 1057 1101 1114 1116 1097 23.8 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 83 84 87 83 2.16 2.6% Sample 1 81 83 84 87 83 2.16 2.6% Sample 2 485 539 463 476 490 28.93 5.9% Sample 2 485 539 463 476 490 28.93 5.9% Sample 3 1057 1101 1114 1116 1097 23.8 2.1% Sample 3 1057 1101 1114 1116 1097 23.8 2.1%

Atherosclerosis, Cancer, Cardiovascular, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Mesenchymal Stem Cells, Metabolism, Neurology Process, Neuroscience, Obesity, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 485 1057 75 444 996 Mean (pg/ml) 81 485 1057 75 444 996 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 Standard deviation 6.15 32.49 54.96 8 35.52 69.72 CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7% CV (%) 7.6% 6.7% 5.2% 8.4% 8% 7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497 O.D. 0.007 0.051 0.099 0.207 0.45 0.934 1.769 2.497

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.