Rat Laminin ELISA Kit PicoKine®

Rat Laminin ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Laminin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 256 282 284 258 270 13.03 4.8% Sample 2 1435 1315 1498 1568 1454 93.02 6.3% Sample 3 4770 4867 4216 4900 4688 276.8 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 256 282 284 258 270 13.03 4.8% Sample 1 256 282 284 258 270 13.03 4.8% Sample 2 1435 1315 1498 1568 1454 93.02 6.3% Sample 2 1435 1315 1498 1568 1454 93.02 6.3% Sample 3 4770 4867 4216 4900 4688 276.8 5.9% Sample 3 4770 4867 4216 4900 4688 276.8 5.9%

Synonyms

Laminin subunit beta-2; Laminin chain B3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta; Lamb2

Gene Name

LAMA1; LAMA2; LAMB1; LAMB2

UniProt

D4A409, F1M614, D3ZN05, F1LTF8, F1MAN8, D3ZQN7, P15800, F1LPI5, F1MAA7, F1LRH4, A0A0G2K023

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Target protein isolated from rat sarcoma basement membrane

Capture Antibody

Polyclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Found in the basement membranes (major component) . S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 256 1435 4770 279 1524 4389 Standard deviation 11.26 97.58 352.98 15.34 128.01 373.06 CV (%) 4.4% 6.8% 7.4% 5.5% 8.4% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 256 1435 4770 279 1524 4389 Standard deviation 11.26 97.58 352.98 15.34 128.01 373.06 CV (%) 4.4% 6.8% 7.4% 5.5% 8.4% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 256 1435 4770 279 1524 4389 Mean (pg/ml) 256 1435 4770 279 1524 4389 Standard deviation 11.26 97.58 352.98 15.34 128.01 373.06 Standard deviation 11.26 97.58 352.98 15.34 128.01 373.06 CV (%) 4.4% 6.8% 7.4% 5.5% 8.4% 8.5% CV (%) 4.4% 6.8% 7.4% 5.5% 8.4% 8.5%

Sensitivity

<10 pg/mL

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

196474 MW

Specificity

Natural rat Laminin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Laminin subunit beta-2

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Contains 13 laminin EGF-like domains.

Protein Name

Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.103 0.176 0.332 0.554 0.935 1.423 1.932 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.103 0.176 0.332 0.554 0.935 1.423 1.932 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.103 0.176 0.332 0.554 0.935 1.423 1.932 O.D. 0.021 0.103 0.176 0.332 0.554 0.935 1.423 1.932

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.