Human Laminin ELISA Kit PicoKine®

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 318 364 314 373 342 26.47 7.7% Sample 2 1051 1242 1122 1215 1157 75.9 6.5% Sample 3 4462 4109 4606 4544 4430 192.37 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 318 364 314 373 342 26.47 7.7% Sample 1 318 364 314 373 342 26.47 7.7% Sample 2 1051 1242 1122 1215 1157 75.9 6.5% Sample 2 1051 1242 1122 1215 1157 75.9 6.5% Sample 3 4462 4109 4606 4544 4430 192.37 4.3% Sample 3 4462 4109 4606 4544 4430 192.37 4.3%

Laminin subunit alpha-1; Laminin A chain; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-laminin subunit alpha; Laminin subunit beta-1; Laminin B1 chain; Laminin subunit gamma-1; LAMB2

Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 318 1051 4462 295 1015 4289 Standard deviation 23.21 47.29 348.03 26.25 58.87 411.74 CV (%) 7.3% 4.5% 7.8% 8.9% 5.8% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 318 1051 4462 295 1015 4289 Standard deviation 23.21 47.29 348.03 26.25 58.87 411.74 CV (%) 7.3% 4.5% 7.8% 8.9% 5.8% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 318 1051 4462 295 1015 4289 Mean (pg/ml) 318 1051 4462 295 1015 4289 Standard deviation 23.21 47.29 348.03 26.25 58.87 411.74 Standard deviation 23.21 47.29 348.03 26.25 58.87 411.74 CV (%) 7.3% 4.5% 7.8% 8.9% 5.8% 9.6% CV (%) 7.3% 4.5% 7.8% 8.9% 5.8% 9.6%

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.097 0.135 0.241 0.47 0.832 1.349 1.833 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.097 0.135 0.241 0.47 0.832 1.349 1.833 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.097 0.135 0.241 0.47 0.832 1.349 1.833 O.D. 0.022 0.097 0.135 0.241 0.47 0.832 1.349 1.833

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.