Human IL-17/IL-17A ELISA Kit PicoKine®

IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13) . IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor, and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 62 58 57 3.31 5.8% Sample 2 276 273 283 261 273 7.94 2.9% Sample 3 763 768 854 781 791 36.67 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 62 58 57 3.31 5.8% Sample 1 54 54 62 58 57 3.31 5.8% Sample 2 276 273 283 261 273 7.94 2.9% Sample 2 276 273 283 261 273 7.94 2.9% Sample 3 763 768 854 781 791 36.67 4.6% Sample 3 763 768 854 781 791 36.67 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 276 763 52 257 703 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 CV (%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 276 763 52 257 703 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 CV (%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 276 763 52 257 703 Mean (pg/ml) 54 276 763 52 257 703 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 CV (%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4% CV (%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4%

Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.11 0.156 0.244 0.457 0.868 1.677 2.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.11 0.156 0.244 0.457 0.868 1.677 2.955 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.11 0.156 0.244 0.457 0.868 1.677 2.955 O.D. 0.056 0.11 0.156 0.244 0.457 0.868 1.677 2.955

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.