Human IL-16 ELISA Kit PicoKine®

Human IL-16 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL16 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 64 55 56 58 3.5 6% Sample 2 213 197 219 232 215 12.57 5.8% Sample 3 983 978 906 908 943 36.79 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 64 55 56 58 3.5 6% Sample 1 59 64 55 56 58 3.5 6% Sample 2 213 197 219 232 215 12.57 5.8% Sample 2 213 197 219 232 215 12.57 5.8% Sample 3 983 978 906 908 943 36.79 3.9% Sample 3 983 978 906 908 943 36.79 3.9%

Synonyms

Pro-interleukin-16; Interleukin-16; IL-16; Lymphocyte chemoattractant factor; LCF; IL16

Gene Name

IL16

Gene ID

3603

UniProt

Q14005

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P1204-S1332

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 213 983 58 211 1038 Mean (pg/ml) 59 213 983 58 211 1038 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 Standard deviation 2.41 14.48 49.15 2.37 18.14 59.16 CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7% CV (%) 4.1% 6.8% 5% 4.1% 8.6% 5.7%

Sensitivity

<10 pg/mL

Function

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

141752 MW

Specificity

Natural and recombinant human IL-16

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Pro-interleukin-16

Subcellular Location

Interleukin-16: Secreted.

Sequence Similarities

Contains 4 PDZ (DHR) domains.

Protein Name

Pro-interleukin-16

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655 O.D. 0.0 0.089 0.163 0.294 0.522 0.945 1.585 2.655

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.