Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% Sample 3 234 249 255 221 239 13.25 5.5%

Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8) 77; GCP/IL-8 protein II; IL-8 (1-77) ; IL8/NAP1 form II; MDNCF-b; IL-8 (5-77) ; IL-8 (6-77) ; (Ser-IL-8) 72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77) ; GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77) ; GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77) ; GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 66 234 16 63 230 Mean (pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6% CV (%) 4.5% 4.3% 6.1% 5% 6.1% 7.6%

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095 O.D. 0.022 0.066 0.106 0.199 0.362 0.681 1.25 2.095

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.