Rat IL-6/Interleukin-6 ELISA Kit PicoKine®

Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH) . Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 192 175 177 184 8.28 4.5% Sample 2 579 631 610 577 599 22.52 3.7% Sample 3 1531 1470 1340 1471 1453 69.76 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 192 175 177 184 8.28 4.5% Sample 1 193 192 175 177 184 8.28 4.5% Sample 2 579 631 610 577 599 22.52 3.7% Sample 2 579 631 610 577 599 22.52 3.7% Sample 3 1531 1470 1340 1471 1453 69.76 4.8% Sample 3 1531 1470 1340 1471 1453 69.76 4.8%

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 579 1531 210 564 1466 Standard deviation 9.65 35.89 90.32 11.55 40.04 109.95 CV (%) 5% 6.2% 5.9% 5.5% 7.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 579 1531 210 564 1466 Standard deviation 9.65 35.89 90.32 11.55 40.04 109.95 CV (%) 5% 6.2% 5.9% 5.5% 7.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 193 579 1531 210 564 1466 Mean (pg/ml) 193 579 1531 210 564 1466 Standard deviation 9.65 35.89 90.32 11.55 40.04 109.95 Standard deviation 9.65 35.89 90.32 11.55 40.04 109.95 CV (%) 5% 6.2% 5.9% 5.5% 7.1% 7.5% CV (%) 5% 6.2% 5.9% 5.5% 7.1% 7.5%

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.117 0.194 0.296 0.525 0.919 1.523 1.982 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.117 0.194 0.296 0.525 0.919 1.523 1.982 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.117 0.194 0.296 0.525 0.919 1.523 1.982 O.D. 0.051 0.117 0.194 0.296 0.525 0.919 1.523 1.982

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.