Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il5 in cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF) . IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 37 35 34 2.27 6.6% Sample 2 220 228 267 256 242 19.35 7.9% Sample 3 453 402 397 434 421 23.07 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 37 35 34 2.27 6.6% Sample 1 36 31 37 35 34 2.27 6.6% Sample 2 220 228 267 256 242 19.35 7.9% Sample 2 220 228 267 256 242 19.35 7.9% Sample 3 453 402 397 434 421 23.07 5.4% Sample 3 453 402 397 434 421 23.07 5.4%

Synonyms

Interleukin-5; IL-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF; Il5; Il-5

Gene Name

IL5

Gene ID

16191

UniProt

P04401

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M21-G133

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, B Cells, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Non-CD, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4%

Sensitivity

<2 pg/mL

Function

Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15410 MW

Specificity

Natural and recombinant mouse IL-5

Gene Name Synonym

Interleukin-5

Subcellular Location

Secreted.

Protein Name

Interleukin-5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.