Rat IL-4/Interleukin-4 ELISA Kit PicoKine®

Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 30 36 34 2.34 6.8% Sample 2 164 156 144 155 154 7.11 4.6% Sample 3 456 463 512 430 465 29.65 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 30 36 34 2.34 6.8% Sample 1 35 35 30 36 34 2.34 6.8% Sample 2 164 156 144 155 154 7.11 4.6% Sample 2 164 156 144 155 154 7.11 4.6% Sample 3 456 463 512 430 465 29.65 6.3% Sample 3 456 463 512 430 465 29.65 6.3%

Interleukin-4; IL-4; B-cell IGG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1; Il4; Il-4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 164 456 32 175 481 Standard deviation 2.45 10.66 21.43 2.4 11.37 28.37 CV (%) 7% 6.5% 4.7% 7.5% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 164 456 32 175 481 Standard deviation 2.45 10.66 21.43 2.4 11.37 28.37 CV (%) 7% 6.5% 4.7% 7.5% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 164 456 32 175 481 Mean (pg/ml) 35 164 456 32 175 481 Standard deviation 2.45 10.66 21.43 2.4 11.37 28.37 Standard deviation 2.45 10.66 21.43 2.4 11.37 28.37 CV (%) 7% 6.5% 4.7% 7.5% 6.5% 5.9% CV (%) 7% 6.5% 4.7% 7.5% 6.5% 5.9%

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.039 0.094 0.133 0.22 0.406 0.726 1.251 1.799 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.039 0.094 0.133 0.22 0.406 0.726 1.251 1.799 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.039 0.094 0.133 0.22 0.406 0.726 1.251 1.799 O.D. 0.039 0.094 0.133 0.22 0.406 0.726 1.251 1.799

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.