Human IL-4/Interleukin-4 ELISA Kit PicoKine®

Human IL-4/Interleukin-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 34 34 33 1.5 4.5% Sample 2 216 213 203 197 207 7.62 3.6% Sample 3 508 515 448 502 493 26.52 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 34 34 33 1.5 4.5% Sample 1 35 31 34 34 33 1.5 4.5% Sample 2 216 213 203 197 207 7.62 3.6% Sample 2 216 213 203 197 207 7.62 3.6% Sample 3 508 515 448 502 493 26.52 5.3% Sample 3 508 515 448 502 493 26.52 5.3%

Synonyms

Interleukin-4 ; IL-4 ; B-cell stimulatory factor 1 ; Lymphocyte stimulatory factor 1 ; IL4 ; hCG_24113

Gene Name

IL4

Gene ID

3565

UniProt

P05112

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H25-S153

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5%

Sensitivity

<1.5 pg/mL

Function

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17492 MW

Specificity

Natural and recombinant human IL-4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-4

Subcellular Location

Secreted .

Protein Name

Interleukin-4

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.