Human CD25/IL-2sR Alpha ELISA Kit PicoKine®

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 57 51 60 54 1.37 2.6% Sample 2 230 195 151 171 187 11.21 6.0% Sample 3 618 978 662 709 742 57.86 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 57 51 60 54 1.37 2.6% Sample 1 46 57 51 60 54 1.37 2.6% Sample 2 230 195 151 171 187 11.21 6.0% Sample 2 230 195 151 171 187 11.21 6.0% Sample 3 618 978 662 709 742 57.86 7.8% Sample 3 618 978 662 709 742 57.86 7.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 230 618 57 213 775 Standard deviation 2.88 10.88 21.75 2.94 5.64 30.07 CV (%) 6.3% 4.7% 3.5% 5.2% 2.7% 3.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 230 618 57 213 775 Standard deviation 2.88 10.88 21.75 2.94 5.64 30.07 CV (%) 6.3% 4.7% 3.5% 5.2% 2.7% 3.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 230 618 57 213 775 Mean (pg/ml) 46 230 618 57 213 775 Standard deviation 2.88 10.88 21.75 2.94 5.64 30.07 Standard deviation 2.88 10.88 21.75 2.94 5.64 30.07 CV (%) 6.3% 4.7% 3.5% 5.2% 2.7% 3.9% CV (%) 6.3% 4.7% 3.5% 5.2% 2.7% 3.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.108 0.158 0.26 0.463 0.78 1.357 2.098 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.108 0.158 0.26 0.463 0.78 1.357 2.098 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.108 0.158 0.26 0.463 0.78 1.357 2.098 O.D. 0.042 0.108 0.158 0.26 0.463 0.78 1.357 2.098

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.