Human IL-2/Interleukin-2 ELISA Kit PicoKine®

Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Sample 3 484 469 488 480 480 7.08 1.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1%

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.