Human IL1R1/Il 1 Ri ELISA Kit PicoKine®

Interleukin 1 receptor, type I (IL1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA) . It is an important mediator involved in many cytokine induced immune and inflammatory responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 179 191 173 182 7.15 3.9% Sample 2 973 977 935 964 962 16.42 1.7% Sample 3 4770 4715 5460 5318 5065 327.7 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 179 191 173 182 7.15 3.9% Sample 1 188 179 191 173 182 7.15 3.9% Sample 2 973 977 935 964 962 16.42 1.7% Sample 2 973 977 935 964 962 16.42 1.7% Sample 3 4770 4715 5460 5318 5065 327.7 6.4% Sample 3 4770 4715 5460 5318 5065 327.7 6.4%

Interleukin-1 receptor type 1; IL-1R-1; IL-1RT-1; IL-1RT1; CD121 antigen-like family member A; Interleukin-1 receptor alpha; IL-1R-alpha; Interleukin-1 receptor type I; p80; CD121a; Interleukin-1 receptor type 1, membrane form; mIL-1R1; mIL-1RI; Interleukin-1 receptor type 1, soluble form; sIL-1R1; sIL-1RI; IL1R1; IL1R, IL1RA, IL1RT1

Atherosclerosis, Cardiovascular, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7%

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.