Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 10 11 11 10 0.43 4.3% Sample 2 60 54 52 53 54 3.11 5.7% Sample 3 123 119 123 112 119 4.49 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 10 11 11 10 0.43 4.3% Sample 1 11 10 11 11 10 0.43 4.3% Sample 2 60 54 52 53 54 3.11 5.7% Sample 2 60 54 52 53 54 3.11 5.7% Sample 3 123 119 123 112 119 4.49 3.7% Sample 3 123 119 123 112 119 4.49 3.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 60 123 10 58 112 Mean (pg/ml) 11 60 123 10 58 112 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 Standard deviation 0.45 2.88 8.97 0.57 3.24 9.96 CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9% CV (%) 4.1% 4.8% 7.3% 5.7% 5.6% 8.9%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.034 0.096 0.135 0.256 0.471 0.891 1.625 2.24 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.034 0.096 0.135 0.256 0.471 0.891 1.625 2.24 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.034 0.096 0.135 0.256 0.471 0.891 1.625 2.24 O.D. 0.034 0.096 0.135 0.256 0.471 0.891 1.625 2.24

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.