Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 16 13 15 1.22 8.1% Sample 2 63 68 60 67 64 3.2 5% Sample 3 151 139 139 146 143 5.06 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 16 13 15 1.22 8.1% Sample 1 15 16 16 13 15 1.22 8.1% Sample 2 63 68 60 67 64 3.2 5% Sample 2 63 68 60 67 64 3.2 5% Sample 3 151 139 139 146 143 5.06 3.5% Sample 3 151 139 139 146 143 5.06 3.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.