Mouse IGFBP-3 ELISA Kit PicoKine®

Insulin-like growth factor (IGF) -binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP) -3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 269 246 224 238 244 16.31 6.6% Sample 2 1078 1247 1209 1042 1144 86.01 7.5% Sample 3 5499 5733 5821 5121 5543 270.83 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 269 246 224 238 244 16.31 6.6% Sample 1 269 246 224 238 244 16.31 6.6% Sample 2 1078 1247 1209 1042 1144 86.01 7.5% Sample 2 1078 1247 1209 1042 1144 86.01 7.5% Sample 3 5499 5733 5821 5121 5543 270.83 4.8% Sample 3 5499 5733 5821 5121 5543 270.83 4.8%

CDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3

Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes-associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 269 1078 5499 266 1016 5861 Standard deviation 13.98 55.8%1 335.43 15.42 61.97 380.96 CV (%) 5.2% 5.4% 6.1% 5.8% 6.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 269 1078 5499 266 1016 5861 Standard deviation 13.98 55.8%1 335.43 15.42 61.97 380.96 CV (%) 5.2% 5.4% 6.1% 5.8% 6.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 269 1078 5499 266 1016 5861 Mean (pg/ml) 269 1078 5499 266 1016 5861 Standard deviation 13.98 55.8%1 335.43 15.42 61.97 380.96 Standard deviation 13.98 55.8%1 335.43 15.42 61.97 380.96 CV (%) 5.2% 5.4% 6.1% 5.8% 6.1% 6.5% CV (%) 5.2% 5.4% 6.1% 5.8% 6.1% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.128 0.248 0.458 0.817 1.452 2.011 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.128 0.248 0.458 0.817 1.452 2.011 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.07 0.128 0.248 0.458 0.817 1.452 2.011 O.D. 0.016 0.07 0.128 0.248 0.458 0.817 1.452 2.011

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.