Human IGFBP-3 ELISA Kit PicoKine®

Insulin-like growth factor (IGF) -binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP) -3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 271 288 290 253 275 14.94 5.4% Sample 2 1607 1578 1461 1560 1551 54.87 3.5% Sample 3 5480 5896 5868 5913 5789 179.26 3% Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 271 288 290 253 275 14.94 5.4% Sample 1 271 288 290 253 275 14.94 5.4% Sample 2 1607 1578 1461 1560 1551 54.87 3.5% Sample 2 1607 1578 1461 1560 1551 54.87 3.5% Sample 3 5480 5896 5868 5913 5789 179.26 3% Sample 3 5480 5896 5868 5913 5789 179.26 3%

CDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ; cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3

Angiogenesis, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes-associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 271 1607 5480 253 1639 5548 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8 CV (%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 271 1607 5480 253 1639 5548 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8 CV (%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 271 1607 5480 253 1639 5548 Mean (pg/ml) 271 1607 5480 253 1639 5548 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8 CV (%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9% CV (%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.058 0.112 0.237 0.485 0.923 1.701 2.554 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.058 0.112 0.237 0.485 0.923 1.701 2.554 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.058 0.112 0.237 0.485 0.923 1.701 2.554 O.D. 0.002 0.058 0.112 0.237 0.485 0.923 1.701 2.554

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.