Mouse IGFBP2 ELISA Kit PicoKine®

Mouse IGFBP2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Igfbp2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% Sample 3 3281 3102 2889 3320 3148 170.63 5.4%

Synonyms

Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; mIGFBP-2; Igfbp2; Igfbp-2

Gene Name

IGFBP2

Gene ID

16008

UniProt

P47877

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E35-Q305

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .

Applications

ELISA

Field of Research

Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4%

Sensitivity

<10 pg/mL

Function

Inhibits IGF-mediated growth and developmental rates (By similarity) . IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32847 MW

Specificity

Natural and recombinant mouse IGFBP2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor-binding protein 2

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 2

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.