Human IGFBP2 ELISA Kit PicoKine®

Human IGFBP2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IGFBP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 124 137 116 126 7.56 6% Sample 2 598 645 665 646 638 24.7 3.8% Sample 3 1114 1062 1085 1063 1081 21.15 1.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 124 137 116 126 7.56 6% Sample 1 128 124 137 116 126 7.56 6% Sample 2 598 645 665 646 638 24.7 3.8% Sample 2 598 645 665 646 638 24.7 3.8% Sample 3 1114 1062 1085 1063 1081 21.15 1.9% Sample 3 1114 1062 1085 1063 1081 21.15 1.9%

Synonyms

Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; IGFBP2; BP2, IBP2

Gene Name

IGFBP2

Gene ID

3485

UniProt

P18065

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E40-Q328

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 128 598 1114 133 553 1221 Mean (pg/ml) 128 598 1114 133 553 1221 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 Standard deviation 5.88 47.24 61.27 7.84 54.19 83.02 CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8% CV (%) 4.6% 7.9% 5.5% 5.9% 9.8% 6.8%

Sensitivity

<10 pg/mL

Function

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34814 MW

Specificity

Natural and recombinant human IGFBP2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor-binding protein 2

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 2

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.102 0.144 0.219 0.377 0.74 1.349 1.881 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.102 0.144 0.219 0.377 0.74 1.349 1.881 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.056 0.102 0.144 0.219 0.377 0.74 1.349 1.881 O.D. 0.056 0.102 0.144 0.219 0.377 0.74 1.349 1.881

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.