Mouse IGF-1 ELISA Kit PicoKine®

Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A) . The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP) . Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 79 75 90 79 6.13 7.7% Sample 2 604 579 666 572 605 37.03 6.1% Sample 3 1350 1408 1325 1461 1386 52.73 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 79 75 90 79 6.13 7.7% Sample 1 75 79 75 90 79 6.13 7.7% Sample 2 604 579 666 572 605 37.03 6.1% Sample 2 604 579 666 572 605 37.03 6.1% Sample 3 1350 1408 1325 1461 1386 52.73 3.8% Sample 3 1350 1408 1325 1461 1386 52.73 3.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 604 1350 69 617 1307 Standard deviation 5.85 24.76 93.15 6.69 30.23 92.79 CV (%) 7.8% 4.1% 6.9% 9.7% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 604 1350 69 617 1307 Standard deviation 5.85 24.76 93.15 6.69 30.23 92.79 CV (%) 7.8% 4.1% 6.9% 9.7% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 604 1350 69 617 1307 Mean (pg/ml) 75 604 1350 69 617 1307 Standard deviation 5.85 24.76 93.15 6.69 30.23 92.79 Standard deviation 5.85 24.76 93.15 6.69 30.23 92.79 CV (%) 7.8% 4.1% 6.9% 9.7% 4.9% 7.1% CV (%) 7.8% 4.1% 6.9% 9.7% 4.9% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.069 0.126 0.239 0.441 0.834 1.441 2.086 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.069 0.126 0.239 0.441 0.834 1.441 2.086 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.069 0.126 0.239 0.441 0.834 1.441 2.086 O.D. 0.019 0.069 0.126 0.239 0.441 0.834 1.441 2.086

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.