Human ICAM-1 / CD54 ELISA Kit PicoKine®

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 260 271 261 266 5.52 2% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 3 4746 4742 4337 4571 4599 166.94 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 260 271 261 266 5.52 2% Sample 1 272 260 271 261 266 5.52 2% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 3 4746 4742 4337 4571 4599 166.94 3.6% Sample 3 4746 4742 4337 4571 4599 166.94 3.6%

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2%

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.