Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 159 165 152 161 6.41 3.9% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 3 4183 3905 4308 3657 4013 252.15 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 169 159 165 152 161 6.41 3.9% Sample 1 169 159 165 152 161 6.41 3.9% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 2 1246 1103 1132 1240 1180 63.61 5.3% Sample 3 4183 3905 4308 3657 4013 252.15 6.2% Sample 3 4183 3905 4308 3657 4013 252.15 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 169 1246 4183 168 1364 4239 Mean (pg/ml) 169 1246 4183 168 1364 4239 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 Standard deviation 9.8 87.22 267.71 9.91 118.66 313.68 CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4% CV (%) 5.8% 7% 6.4% 5.9% 8.7% 7.4%

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.082 0.142 0.273 0.523 1.03 1.741 2.466 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.082 0.142 0.273 0.523 1.03 1.741 2.466 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.024 0.082 0.142 0.273 0.523 1.03 1.741 2.466 O.D. 0.024 0.082 0.142 0.273 0.523 1.03 1.741 2.466

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.