Human Gp130/IL6ST ELISA Kit PicoKine®

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein?which is the founding member of the class of all?cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130?is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound?gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 203 198 207 198 7.88 3.9% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 3 3384 3507 3537 3330 3439 85.34 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 203 198 207 198 7.88 3.9% Sample 1 186 203 198 207 198 7.88 3.9% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 3 3384 3507 3537 3330 3439 85.34 2.4% Sample 3 3384 3507 3537 3330 3439 85.34 2.4%

Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; IL6ST

Adapters, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Type Markers, Cytokines, Hypertrophy, Immunology, Intracellular, Signal Transduction, Transmembrane

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3%

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity) . The type I OSM receptor is capable of transducing OSM-specific signaling events. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.