Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine®

Human GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine® (96 Tests) . Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 76 77 70 73 3.53 4.8% Sample 2 264 272 267 262 266 3.76 1.4% Sample 3 855 943 952 812 890 59.07 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 76 77 70 73 3.53 4.8% Sample 1 69 76 77 70 73 3.53 4.8% Sample 2 264 272 267 262 266 3.76 1.4% Sample 2 264 272 267 262 266 3.76 1.4% Sample 3 855 943 952 812 890 59.07 6.6% Sample 3 855 943 952 812 890 59.07 6.6%

Synonyms

Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF; GDNF

Gene Name

GDNF

Gene ID

2668

UniProt

P39905

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R109-I211

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Applications

ELISA

Field of Research

Developmental Biology, Growth and Development, Neurodegenerative Disease, Neurology Process, Neuroscience, Organogenesis

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 264 855 66 242 810 Mean (pg/ml) 69 264 855 66 242 810 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 Standard deviation 4.96 17.16 36.76 6 16.45 37.26 CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6% CV (%) 7.2% 6.5% 4.3% 9.1% 6.8% 4.6%

Sensitivity

<4 pg/mL

Function

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23720 MW

Specificity

Natural and recombinant human GDNF

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Glial cell line-derived neurotrophic factor

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the TGF-beta family. GDNF subfamily.

Protein Name

Glial cell line-derived neurotrophic factor

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.081 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.081 0.123 0.212 0.336 0.609 1.031 1.798 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.081 0.123 0.212 0.336 0.609 1.031 1.798 O.D. 0.03 0.081 0.123 0.212 0.336 0.609 1.031 1.798

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.