Human G-CSF ELISA Kit PicoKine®

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 57 60 56 58 2.38 4.1% Sample 2 237 209 236 225 226 11.27 4.9% Sample 3 1001 964 888 983 959 43.02 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 57 60 56 58 2.38 4.1% Sample 1 62 57 60 56 58 2.38 4.1% Sample 2 237 209 236 225 226 11.27 4.9% Sample 2 237 209 236 225 226 11.27 4.9% Sample 3 1001 964 888 983 959 43.02 4.4% Sample 3 1001 964 888 983 959 43.02 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2%

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.