Human CXCL6/GCP2 ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2) . As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 54 57 57 56 1.29 2.3% Sample 2 297 302 286 282 291 8.07 2.7% Sample 3 818 857 830 896 850 29.95 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 54 57 57 56 1.29 2.3% Sample 1 57 54 57 57 56 1.29 2.3% Sample 2 297 302 286 282 291 8.07 2.7% Sample 2 297 302 286 282 291 8.07 2.7% Sample 3 818 857 830 896 850 29.95 3.5% Sample 3 818 857 830 896 850 29.95 3.5%

C-X-C motif chemokine 6; Chemokine alpha 3; CKA-3; Granulocyte chemotactic protein 2; GCP-2; Small-inducible cytokine B6; Small-inducible cytokine B6, N-processed variant 1; Small-inducible cytokine B6, N-processed variant 2; Small-inducible cytokine B6, N-processed variant 3; CXCL6; GCP2, SCYB6

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 297 818 55 310 882 Standard deviation 2.39 19.3 39.26 2.53 20.15 51.15 CV (%) 4.2% 6.5% 4.8% 4.6% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 297 818 55 310 882 Standard deviation 2.39 19.3 39.26 2.53 20.15 51.15 CV (%) 4.2% 6.5% 4.8% 4.6% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 297 818 55 310 882 Mean (pg/ml) 57 297 818 55 310 882 Standard deviation 2.39 19.3 39.26 2.53 20.15 51.15 Standard deviation 2.39 19.3 39.26 2.53 20.15 51.15 CV (%) 4.2% 6.5% 4.8% 4.6% 6.5% 5.8% CV (%) 4.2% 6.5% 4.8% 4.6% 6.5% 5.8%

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2) . In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram- negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.203 0.336 0.609 1.043 1.898 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.203 0.336 0.609 1.043 1.898 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.076 0.123 0.203 0.336 0.609 1.043 1.898 O.D. 0.03 0.076 0.123 0.203 0.336 0.609 1.043 1.898

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.