Rat Fractalkine/CX3CL1 ELISA Kit PicoKine®

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice) . CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 448 452 401 450 437 21.26 4.8% Sample 2 1815 1598 1565 1811 1697 116.34 6.8% Sample 3 9967 9196 9761 9169 9523 348.57 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 448 452 401 450 437 21.26 4.8% Sample 1 448 452 401 450 437 21.26 4.8% Sample 2 1815 1598 1565 1811 1697 116.34 6.8% Sample 2 1815 1598 1565 1811 1697 116.34 6.8% Sample 3 9967 9196 9761 9169 9523 348.57 3.6% Sample 3 9967 9196 9761 9169 9523 348.57 3.6%

Fractalkine; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1; Processed fractalkine; Cx3cl1; Acc1, Fkn, Scyd1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 448 1815 9967 464 1947 10491 Standard deviation 32.7 76.23 687.72 35.72 87.61 912.71 CV (%) 7.3% 4.2% 6.9% 7.7% 4.5% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 448 1815 9967 464 1947 10491 Standard deviation 32.7 76.23 687.72 35.72 87.61 912.71 CV (%) 7.3% 4.2% 6.9% 7.7% 4.5% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 448 1815 9967 464 1947 10491 Mean (pg/ml) 448 1815 9967 464 1947 10491 Standard deviation 32.7 76.23 687.72 35.72 87.61 912.71 Standard deviation 32.7 76.23 687.72 35.72 87.61 912.71 CV (%) 7.3% 4.2% 6.9% 7.7% 4.5% 8.7% CV (%) 7.3% 4.2% 6.9% 7.7% 4.5% 8.7%

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.079 0.116 0.207 0.338 0.569 1.051 1.829 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.079 0.116 0.207 0.338 0.569 1.051 1.829 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.079 0.116 0.207 0.338 0.569 1.051 1.829 O.D. 0.031 0.079 0.116 0.207 0.338 0.569 1.051 1.829

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.